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. 2012 May 25;7(5):e37936. doi: 10.1371/journal.pone.0037936

Figure 1. Cap-independent translation of mPer1.

Figure 1

(A) Rapamycin (Rapa) or cycloheximide (CHX)-treated NIH 3T3 cells were harvested at indicated time points; then the protein levels were checked by immunoblotting. (B, C, D and E) Vehicle (DMSO)-, rapamycin (Rapa)-, or cycloheximide (CHX)-treated NIH 3T3 cells were harvested at the indicated time points; then mRNA levels were checked by real-time PCR with specific primers, (B) mPer1, (C) mActb, (D) mGapdh and (E) mRpl32. mRNA levels were shown as cycle threshold (Ct) value.

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