Table 1. Differentially expressed proteins identified by DIGE and MS.
DIGE spot no. | Protein description | Species | GI no. | Function | Subcellular location | Theoretical MW (Da)/pI | ExperimentalMW (kDa)/pI | Peptide Count | Protein Score | Protein ScoreC.I % |
301 | ATP synthase CF1-α subunit | Nicotiana sylvestris | gi|78102516 | energy metabolism | mitochondrial membranes | 55388/5.14 | 62.4/5.15 | 19 | 686 | 100 |
178 | Chain A of ribulose-1,5-bisphosphate carboxylase oxygenase | N. tabacum | gi|515239 | photosynthesis and photorespiration | chloroplast | 49526.1/6.19 | 70.1/6.57 | 14 | 250 | 100 |
457 | putative β-galactosidase | Solanum lycopersicum | gi|7939623 | carbon metabolism | lysosome | 93183.9/6.81 | 46.9/7.28 | 11 | 339 | 100 |
598 | carboxylase/oxygenase | N. tabacum | gi|223593 | carbon metabolism | cytoplasm | 14490.2/4.99 | 40.5/5.05 | 5 | 65 | 78.524 |
769 | HrBP1 | N. tabacum | gi|38679323 | disease resistance | cell membrane | 29980/8.8 | 34.4/4.66 | 8 | 242 | 100 |
990 | germin-like protein | N. tabacum | gi|222051768 | disease resistance | cytoplasm | 21408.2/5.84 | 28.2/5.77 | 4 | 257 | 100 |
1015 | oxygen-evolving complex33-kDa photosystem II | N. tabacum | gi|30013657 | photosynthesis and photorespiration | chloroplast | 35176.9/5.63 | 27.9/6.17 | 11 | 284 | 100 |
1041 | 24 K germin-like protein | N. tabacum | gi|31711507 | disease resistance | cytoplasm | 21954.4/7.82 | 27.1/7.78 | 4 | 350 | 100 |
187 | truncated N-protein | N. tabacum | gi|45544515 | disease resistance | cytoplasm | 74429 | 70.15/5.53 | 30 | 4.9e+02 | 4 |
210 | putative cell cycle protein | N. tabacum | gi|82775180 | cell division | nucleus | 20523 | 68.55/7.28 | 22 | 2.8e+03 | 2 |