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. 2012 May 25;7(5):e37944. doi: 10.1371/journal.pone.0037944

Table 5. Primer sequences used in semi-quantitative RT-PCR and qPCR.

Spot no. GI Gene name Forward primer Reverse primer Product (bp)
178 U35619.1 bisphosphate carboxylase oxygenase 5′-gtgcaaacattttggcaatg-3′ 5′-attcagcaactgcatctgga-3′ 178
187 U15605.1 truncated N protein 5′-ggggagtcggtaaaacaaca-3′ 5′-cagagagaagggcattttgc-3′ 153
210 AJ866275.1 putative cell cycle protein 5′-tggtaccgggaacttcagtc-3′ 5′-tggtcacagagctccatttg-3′ 228
301 NC_001879 ATP synthase CF1 alpha subunit 5′-aaaagggcttctgcttcctc-3′ 5′-aaaagggcttctgcttcctc-3′ 153
422 Z14980.1 ribulose-bisphosphate carboxylase activase 5′-cggagaattggaaagtggaa-3′ 5′-acgtttgtcgggttatcagc-3′ 219
457 AJ250431.1 beta-galactosidase 5′-agcctggaactgattgtgct-3′ 5′-tggttgggactttttcttgg-3′ 240
598 gi|223593 carboxylase/oxygenase 5′-tcagatcaagggcattttcc-3′ 5′-caagcgccatatcagcatta-3′ 240
701 DQ442995 Phosphomannomutase 5′-agcacctcgcaaggaagtta-3′ 5′-tccttgagcttctcgtctcc-3′ 240
769 AY383625.1 harpin binding protein 5′-aatttgccagtcgggtattg-3′ 5′-caatttccacctcccttgaa-3′ 247
990 AB449366 germin like protein 5′-ggcttagtgcagctggaaac-3′ 5′-cagggtgagtgtgaaatgga-3′ 153
1015 AY220076.1 oxygen evolving complex photosystem II 5′-acctgcagttgaggtctgct-3′ 5′-catttgctcctgagacgaca-3′ 180
1041 gi|31711507 24 K germin-like protein 5′-cgatttctgtgttggggact-3′ 5′-ggtgtgactgcggctttaat-3′ 150
5942 gi|12643758 ribulose bisphosphate carboxylase/oxygenase-2(RuBisCO activase) 5′-tgttcctgctgaagacgttg-3′ 5′-agttggtggtccgtcaaaag-3′ 175
6071 J02979.1 lignin-forming peroxidase 5′-agcccttgcatctgaaattg-3′ 5′-ttccaaatgtgtgtgcacct-3′ 215
6095 gi|255542955 NAD-dependent epimerase/dehydratase 5′-gcaggcaaaaggagcactac-3′ 5′-ggtctgattcacctggcaat-3′ 179
6138 XM_002520879 stability/assembly factor HCF 136 5′-acatccaatgggggatacaa-3′ 5′-cggcttgagacagcaacata-3′ 164
6257 Y15489 plastid-lipid-associated protein 5′-agcagaggaagaaccaccaa-3′ 5′-ctcggtaggagcaggagttg-3′ 178
6288 AY220076.1 complex 33-kDa photosystem-II protein 5′-acctgcagttgaggtctgct-3′ 5′-catttgctcctgagacgaca-3′ 180
6388 AM087457.1 chloroplast cysteine synthase 1 5′-cttattgttgccgtggacct-3′ 5′-gcaatgtttgcaacacaacc-3′ 157
6449 DQ222496.1 cytosolic isoform of triosephosphate isomerase 5′-gttgtgagccctccatttgt-3′ 5′-ccaaaattacccacggaatg-3′ 163
6527 gi|52000814 oxygen-evolving enhancer protein 2-1 5′-gtagcgatcgccttcttgac-3′ 5′-ttggggttaataggggaagg-3′ 204
6545 AB120535 CDK-5 regulatory subunit associated 5′-gtcaagcaaaggaggctcac-3′ 5′-atgacaccctatggcctctg-3′ 169
6556 X64198 photosystem I light-harvesting chlorophylla/b-binding protein 5′-gtttccccctctgtcctctc-3′ 5′-gggcagaaccatcaaggtaa-3′ 151
6563 AB093097 Superoxide dismutase 5′-agctacatgacgccatttcc-3′ 5′-ccctgtaaagcagcaccttc-3′ 238
6675 D13718.1 PSI-D1 precursor 5′-ctcaaattggcccgtaaaga-3′ 5′-tgaaattctgcccaactcct-3′ 181
6883 AY220079.1 carboxylase small subunit precursor protein 5′-catggttgcacctttcactg-3′ 5′-ttccaaacaaggaacccatc-3′ 238
AF480488 NPR1 5′-tagcgtattgcgatgcaaag-3′ 5′-ttccatcggatgtcagatca-3′ 180
AF480489 EDS1 5′-aggccgaagcgttataggtt-3′ 5′-aaaacatcatcgcccagaag-3′ 203
X05959.1 PR-1a 5′-caatacggcgaaaacctagctga -3′ 5′- cctagcacatccaacacgaa-3′ 170
X03913 PR-5 5′-gcttccccttttatgccttc-3′ 5′-cctgggttcacgttaatgct-3′ 150
reference gene GQ339768.1 β-actin 5′-ttgtccgtgacatgaaggag-3′ 5′-atcatggatggctggaagag-3′ 178
reference gene AF120093.1 EF-1a 5′-ctctcaggctcccacttcag-3′ 5′-aagagcttcgtggtgcatct-3′ 164
reference gene HQ384692.1 18S ribosomal RNA gene 5′-atggccgttcttagttggtg-3′ 5′-tgtcggccaaggctataaac-3′ 235