Figure 4. PARP inhibition reduces the transcriptional induction of anthocyanin pathway genes.

Arabidopsis plants (Col-0) were grown at 80–100 µE, 22°C on MS medium with (+3 MB) or without (−3 MB) the PARP inhibitor 3-Methoxy-benzamide (3 MB) and subjected to three different conditions: control, oxidative stress (0.1 µM Paraquat) or sucrose stress (150 mM Sucrose) and harvested after 14 days. RNA was extracted from seedlings pooled from all five replicates within one experiment, with three independent experiments (n = 3). The average relative expression, normalized against the housekeeping gene PP2A, is shown, for the biosynthetic (A–F) and the regulatory (G–I) genes.