Table 1. Metabolite changes induced by PARP inhibition and detected by GC-MS.
Metabolite | Fold-change (+3 MB) |
Arabinose | 1.78 |
Ethanolamin | 0.82 |
Fumarate | 0.46 |
Galactinol | 0.58 |
Galactonic acid | 0.82 |
Myo-inositol | 0.65 |
Sinapic acid | 0.45 |
For the GC-MS metabolite profiling samples, a total of ∼10 seedlings from 4 plates were harvested individually for both conditions (+/−3 MB) in each experiment, repeated in three individual experiments (n = 12). The plants were grown in parallel to those for the microarray expression analysis. The metabolite extraction and further processing was done at the “Plateforme Métabolisme-Métabolome (IFR87)” accordingly to (55). Metabolites that were significantly different (t-test, p<0.05, Bonferroni-Holm multiple testing correction) between the 3 MB treated plants (+3 MB) and the control plants (−3 MB) are shown. Values presented are the ratio (fold-change) in the abundance of +3 MB relative to −3 MB.