Figure 7.
Aggregate of Bry-GFP ESCs at 75 μm electrode region at zero hours, formed by applying a 1 MHz, 10 Vpk-pk signal to interdigitated oppositely castellated electrodes. The aggregate was immobilized in 25% Puramatrix and incubated with GMEM growth medium without LIF. (a) Green fluorescence image of an aggregate at zero hours showing the region of interest for image analysis. (b) Bright field image of an aggregate at zero hours. (c) Green fluorescent image of an aggregate at 24 h showing the region of interest. (d) Bright field image of an aggregate forming an EB after 24 h.