Figure 2.

Effect of microchannel height (aspect ratio) on bacteria margination. (a) Bacteria concentration in the filtered centre outlet with varying channel heights using a single margination channel of 15 mm length and 20 µm width (schematic single channel design in red box). Bacteria concentration was normalized with sample to determine the percentage of bacteria remaining after filtration. (b) Fluorescent intensity Z-profiles at the outlet centre region of 20 µm and 75 µm height channels and corresponding schematic illustration of their bacteria margination. Confocal images at the mid plane of 20 µm and 75 µm height channels indicate less efficient bacteria margination at the mid plane region of high aspect ratio channel.