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. 2012 May 25;7(5):e36109. doi: 10.1371/journal.pone.0036109

Figure 2. BPA differentially regulates the mRNA expression of adipogenic marker genes during adipogenesis.

Figure 2

hASCs were induced to differentiate with an optimised adipocyte differentiation medium in the presence of 80 µM BPA or vehicle. qRT-PCR was performed on days 3, 5, 9, 11 and 14 of differentiating cells treated with vehicle or BPA using specific primer pairs for PPAR γ (Ai), C/EBP α (Aii), LPL (Aiii), aP2 (Aiv) and FAS (Av). The relative qRT-PCR values were corrected to GAPDH expression levels and normalized with respect to vehicle controls on each day. Values are mean ± S.D. of three independent experiments. *p<0.05, ** p<0.001, *** P<0.0001 as compared with vehicle control for each day. (B) To show the kinetics in gene expression throughout adipogenesis one representative experiment from A is corrected to GAPDH expression levels and normalized to non-induced day 0 cells for PPAR γ (Bi) and C/EBP α (Bii) and FAS (Bv). Expression of LPL and aP2 was negligible in day 0 cells and so data was normalised to day 3 vehicle treated cells for LPL (Biii) and aP2 (Biv).