Figure 6. LPL overexpression differentially regulates the mRNA expression of adipogenic marker genes during adipogenesis in response to BPA.

hASCs transduced with control (pCDH) or LPL expressing (pCDH-LPL) lentiviral expression vectors were induced to differentiate with adipocyte differentiation media in the presence of 80 µM BPA. On day 10 of differentiation total RNA was isolated and reverse transcribed. qPCR was performed using specific primer pairs for PPAR γ, C/EBP α, and aP2. The relative qPCR values were corrected to GAPDH expression levels and normalized with respect to pCDH controls on each day. Values are the mean ± SD of three independent experiments.