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. 2012 May 25;7(5):e36861. doi: 10.1371/journal.pone.0036861

Figure 4. Morphometry of astrocytic processes in the hippocampus.

Figure 4

(A) Confocal analysis of GFAP immunofluorescence was performed in the DG, the CA1 and the CA3 regions of the hippocampus of 3-month old (young) and 22-month old (aged) mice fed with the control diet or the LCω3 diet for 2 months using a 63X oil-immersion lens with a 3.10X zoom and IMARIS software. Images are representative of GFAP 3D immunofluorescence in the DG (upper panel), CA1 (central panel) and CA3 (lower panel). Scale bar = 8.5 µm. (A) Morphometric analysis of astrocytes in the DG, CA1 and CA3 regions of the hippocampus was performed using the “filament tracer” program/function. Data present means of primary and secondary processes lengths expressed in µm ± SEM. *** p<0.001, ** p<0.01, * p<0.05.