Figure 4. Recognition of M.cat by host depends on combined effect of TLR2/4/9.

BMMØ were generated from TLR2/4/9 triple knock-out and WT control mice (C57BL/6J) and cultured in 24 well tissue culture plate at 2×10⁵ cells/well. Cells were then treated with LOS (100 ng/ml), Pam3 (10 µg/ml), CpG DNA (20 µg/ml), HK and live M.cat for 6 h. Supernatants were collected and the levels of TNF-α (A) and IL-6 (B) were measured by ELISA. Representative data from two independent experiments are shown.