Figure 6. Impaired immune response to M.cat by TRIF-deficient macrophages.
Bone marrow derived macrophages were generated from TRIF knock-out and wild type control mice (C57BL/6J) and cultured in 24 well tissue culture plate at 2×105 cells/well. Cells were then treated with LOS (100 ng/ml), Poly I:C (50 µg/ml), HK and live M.cat for 18 h (A–B) or 6 h (C). A, C, Supernatants were collected and the levels of IFN-β (A) and IL-6 (C) were measured by ELISA. B. Total RNA was extracted after 18 h of M.cat treatment and gene expression of RANTES was analyzed by real time RT-PCR. Representative data from two independent experiments are shown.