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. 2012 May 25;7(5):e36693. doi: 10.1371/journal.pone.0036693

Figure 5. Activation of MAPK pathways in OASF after ATP stimulation.

Figure 5

A. OASF were stimulated with ATP (100 µM) for 10 minutes, 2 h, 5 h and 24 h. Western blot analysis of p38 and p44/42 MAPK, as well as phosphorylated p-p38 and p-p44/42 MAPK showed kinase activation after 10 minutes ATP stimulation. B. OASF (n = 10) and RASF (n = 3) were treated with the kinase inhibitors FR180204, SB203580 and PD98059, respectively 15 min before ATP (100 µM) stimulation for 2 h. Data are shown as means ± standard deviations. *, p<0.05.