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Perithecium centra were fixed, labeled with DAPI, and observed for the presence of croziers. Croziers were observed in all homeobox mutants, as shown here for Δpah1. Some combinations of multiple mutants were also examined and found to differentiate croziers, as exemplified here by the Δpah2Δpah3Δpah4 triple mutant and the Δpah1Δpah2Δpah3Δpah4Δpah5Δpah6 sextuple mutant.
