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. 2012 May 25;7(5):e37564. doi: 10.1371/journal.pone.0037564

Figure 4. The effect of respiratory chain substrates on mitochondrial membrane potential and ROS generaton.

Figure 4

a: Application of 5 mM pyruvate reduced the dopamine induced mitochondrial depolarisation, but did not completely prevent it. b: Dopamine induced a rise in mitochondrial superoxide in PINK1 ko neurons, but not in wt neurons. 5 mM pyruvate and 5 mM Me-succinate both reduced the dopamine-induced mitochondrial ROS production. c: Histogram comparing the reduction of dopamine induced mitochondrial depolarisation by different compounds: the PTP inhibitor CsA, calcium chelator BAPTA-AM, NADPH oxidase inhibitor DPI, mitochondrial antioxidant MitoQ, MAO inhibitor selegiline, respiratory chain substrates pyruvate and me-succinate, and the calcium channel blocker verapamil all resulted in a statistically significant reduction in dopamine induced mitochondrial depolarisation in PINK1 ko neurons.