Fig. 5.

Quercetin inhibits cleavage of eIF4GI, increases phosphorylation of eIF2α and decreases levels of VP2. BEAS-2B cells were infected with RV and incubated for 90 min. Infection medium was replaced with fresh media, incubated for 4.5 h and then quercetin (10 μM) (or DMSO) were added to the cells. After a total of 24 h, total protein was isolated and subjected to Western blot analysis using antibody to eIF4GI (A), eIF2α (B) or VP2 (C). Blots are representative of three independent experiments.