Fig. 7.

Quercetin decreases viral load in vivo. C57BL/6 mice were infected with RV1B or sham by the intranasal route. Two hours after RV infection, mice were orally gavaged with quercetin (0.2 mg/mouse) or 50% propylene glycol (PG) and then once a day up to 4 days. Mice were sacrificed and infectious viral load (A) was determined by measuring CCID₅₀/ml. Positive strand vRNA (B) was determined by qPCR and negative strand vRNA (C) was detected by qPCR followed by agarose gel electrophoresis. (A and B) Data represent geomean and range of data from two independent experiments carried out in triplicate (^†p ⩽ 0.05, different from mice infected with RV1B infected and treated with PG). (C) PCR product from two representative animals from PG and quercetin group showing negative-strand vRNA and 18S RNA.