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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Anesthesiology. 2012 Jun;116(6):1245–1257. doi: 10.1097/ALN.0b013e318255793c

Figure 6. Cytokine profiling during ischemia reperfusion.

Figure 6

(A–D) Wild-type (WT) or Adora2b−/− mice underwent 60 min ischemia followed by 120 min of reperfusion. The area at risk was harvested, homogenized and probed for various cytokines using a muliplex ELISA. Values were normalized to the total protein content of the tissue samples. (A) To quantify polymorphonuclear leukocytes (PMN) sequestration into the myocardium, an ELISA for Myeloperoxidase (MPO) was performed (WT: 1.4 ± 0.2 ng/mg Protein; Adora2b−/−: 1.5 ± 0.1 ng/mg protein; not significant). (B, C) In order to characterize the inflammatory response in WT and Adora2b−/−, Interferon-γ (Inf-γ), Interleukin -1β (IL-1β), Interleukin -2 (IL-2), Interleukin -4 (IL-4), Interleukin -5 (IL-5), Keratinocyte-derived Cytokine (KC), Interleukin - 10 (IL-10) and Interleukin -12 (IL-12) tissue levels were measured; Note: no significant changes were found whereas Interleukin-2 and Interleukin-10 were negative in both groups (data not shown). (D) Tumor necrosis factor-alpha (TNFα) content of the area at risk in WT and Adora2b−/− (WT: 7.5 ± 0.5 pg/mg protein; Adora2b−/−: 39.3 pg/mg protein); mean ± SD; n = 4 per group.