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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Biochem. 2012 Jun;113(6):1998–2008. doi: 10.1002/jcb.24069

Figure 3.

Figure 3

ALOX5 expression and activity under normal culture conditions. (A) Growth curves were generated for ALOX5-BCPAP and pcDNA-BCPAP under standard conditions by trypan-blue exclusion. (B) Ten-thousands cells per condition were grown to 50% confluence and cell cycle was assessed by laser scanning cytometry. (C) The dose dependent effects of arachidonic acid (C) and Zileuton (D) on cell viability were determined by XTT metabolism. Twenty-five thousand cells were plated in replicates of 6 for each condition and cell viability was measured at 72 hours post treatment; error bars represent standard deviation.