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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Biochem. 2012 Jun;113(6):1998–2008. doi: 10.1002/jcb.24069

Figure 5.

Figure 5

MMP-9 levels in BCPAP conditioned media in response to ALOX5 gene transfection and exogenous 5-HETE. (A) Conditioned media were prepared from 100,000 pcDNA-BCPAP and ALOX5-BCPAP cells cultured, in duplicate, and MMP protein levels were assessed using the RayBio Human Matrix Metalloproteinases Antibody Array. ALOX5-BCPAP has significantly higher levels of MMP-9 compared to pcDNA-BCPAP (p=0.032, t-test). (B) 5-HETE and ALOX5 increase MMP-9 levels in conditioned media, determined by western blot analysis.