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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Dig Liver Dis. 2012 Mar 28;44(7):589–596. doi: 10.1016/j.dld.2012.02.016

Figure 2.

Figure 2

miR-21 modulates the expression of Serpini1 and directly interacts with Serpini1 3’UTR.

Panel A. Representative Western blot demonstrating increased protein level of Serpini1 following miR-21 inhibition (21-I in the figure). NSI – non specific inhibitor.

Panel B. MKN28 cells were transfected with a fragment of Serpini1 3’UTR (Serpini1 in the figure) or with a fragment of Serpini1 3’UTR with a mutated miR-21 binding site (Serpini1Mut in the figure), cloned downstream of the luciferase promoter. These cells were co-transfected with either miR-21 inhibitor (21Inh in the figure) or with a non-specific inhibitor (NSI in the figure). miR-21 inhibitor moderately increases the activity of the luciferase compared to the mutated control. Y-axis: arbitrary luminescence units. Standard errors are shown.