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. Author manuscript; available in PMC: 2013 May 22.
Published in final edited form as: Curr Biol. 2012 Apr 26;22(10):872–880. doi: 10.1016/j.cub.2012.03.060

Figure 4.

Figure 4

Two clones share CDR3 and switch junctions. Top, Diagram shows sequences detected by RT-PCR using primers in the J-C intron of G5 and leader of G4. Yellow indicates sequence identity with G4, pink with G5; “ccc” insertion at putative switch point. Not drawn to scale, as indicated by break symbol. Bottom. G4 VDJ joined to G5 intron are compared to G4A and G5 reference sequences. Related clones 4–21 and 4–36 share CDR3 and mutations (blue) through VDJ and JH flank. The flanking intron is labeled. Hotspot motifs RGYW/WRCY are underlined in the reference sequences. Arrow (switch point) indicates where the G4A sequence identity ceases. This is followed nontemplated addition (ccc) and identity with G5 (in pink). Accession numbers JQ272798-99.