Table 1.

A summary of genes that regulate emodepside sensitivity in the nematode C. elegans

Gene	Pharynx	Locomotion	Signalling cascade
slo-1	↓1000 fold	↓1000 fold	Ion channel
lat1/lat2	↓10-fold	No change	GPCR
goa-1	↑10-fold	↑50 fold	G-protein
egl-30 (lof)	↓50-fold	↓3 fold	G-protein
egl-30 (gof)	↑20-fold	↑20 fold	G-protein
egl-8	↓14-fold	↓10 fold	Phospholipase C
unc-13	↓40-fold	–	Transmitter release
unc-10	No change	–	Transmitter release
snb-1	↓30-fold	–	Transmitter release
unc-31	↓5-fold	–	Transmitter release

The sensitivity to emodepside of the C. elegans mutants listed was compared in parallel assays with the sensitivity of wild-type animals and the relative sensitivity of the mutant compared to wild-type expressed as a fold reduction (↓) or a fold increase (↑). ‘lof’ indicates a loss of function mutant and ‘gof’ indicates a gain-of-function mutant. The left-hand column indicates the mutants that were assayed. The effect of emodepside was determined in a pharyngeal assay in which extracellular electrophysiological recordings were made from the pharynx (Willson 2003; Willson et al. 2004; Bull 2007) and on locomotion in which the effect of emodepside on the frequency of locomotion on agar medium was determined (Amliwala 2005)