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. Author manuscript; available in PMC: 2012 Oct 14.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 14;414(1):67–72. doi: 10.1016/j.bbrc.2011.09.023

Figure 1. Expression of PAI-1 is upregulated during NTHi infection both in epithelial cells in vitro and in lung tissues of mice in vivo.

Figure 1

A. NTHi induces PAI-1 mRNA expression in airway epithelial A549 cells. Cells were treated with NTHi or PBS as control for 5 hours, and mRNA expression of PAI-1 was measured by using real time quantitative RT-PCR (Q-PCR) analysis. B. NTHi induces PAI-1 transcription in epithelial cells. Cells were transfected with PAI-1-Luc construct and treated with NTHi or PBS 48 hours after transfection. PAI-1 transcription was measured 5 hours after NTHi treatment by using luciferase assay. C. NTHi induces PAI-1 mRNA expression in lung tissues of wild-type (WT) mice. C57BL/6 mice were intratracheally (i.t.) inoculated with NTHi or saline as a control, and mRNA expression of PAI-1 was measured from the lung tissues of mice inoculated with NTHi or saline by using Q-PCR analysis. D. NTHi induces PAI-1 protein expression in lungs of WT mice. C57BL/6 mice were i.t. Inoculated with NTHi or saline as a control, and expression of total PAI-1 protein was measured from the bronchoalveolar lavage fluid (BALF) of mice inoculated with NTHi or saline by using total PAI-1 antigen ELISA assay kit. The values presented in A-D are Mean ± SD (n=3). *, p<0.05 compared with CON; CON: control.