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. Author manuscript; available in PMC: 2012 Oct 14.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 14;414(1):67–72. doi: 10.1016/j.bbrc.2011.09.023

Figure 3. NTHi induces PAI-1 upregulation via TLR2/MyD88 pathway.

Figure 3

A. NTHi-induced PAI-1 expression is dependent on TLR2/MyD88, but not on TLR4 in vitro in epithelial cells. Cells were transfected with PAI-1-Luc construct with Vector, TLR2 DN, TLR4 DN, or MyD88 DN for 48 hours. Transfected cells were then treated with NTHi or PBS as a control, and PAI-1 transcription was measured 5 hours after NTHi or control treatment by luciferase assay. B & C. NTHi induces PAI-1 expression via TLR2 & MyD88 in vivo in mice. TLR2 KO, MyD88 KO, or their littermate WT control mice were i.t. inoculated with NTHi or saline as a control, and mRNA expression of PAI-1 was measured from the lung tissues of mice 6 hours after NTHi or control inoculation. The values presented in A-C are Mean ± SD (n=3). *, p<0.05 compared with CON; #,p>0.05 compared with NTHi in Vector (A) or WT (B & C). CON: control; WT: wild-type; KO: knock-out; DN; dominant-negative.