Figure 3. Tumor Lysates from 5% oxygen prime CTLs with superior tumoricidal function.

A, PBMCs were stimulated with DCs pulsed with TL from 5% or 20% O₂ and incubated with HLA-A2 matched target glioblastoma cells (Patients 3 and 4) or an ependymoma (Patient 5). B, to determine if the increased tumoricidal response was MHC-I-dependent, pan-MHC I blocking antibody was added to target cells prior combining with PBMC. C, DCs pulsed with TL from 5% or 20% O₂ from patient 3 and incubated with patient 3 target cells cultured in 20% or 5% O₂. All experiments were repeated at least twice with similar results. Error bars, ± SEM. * P < 0.05; ** P < 0.01.