Figure 3. Hair cycles of transplanted regenerated hair follicle ectopically.

(a) The macroscopic analyses of various phases of the hair cycles. The bioengineered hair follicles were observed at 13 days, 17 days, 27 days and 39 days after transplantation. (b) Cycles of hair growth (dark bars) and regression (light bars) phases. The left panel shows the term of growth and regression phases of the individual regenerated hairs until 60 days after transplantation. The right panel shows the hair cycle term of natural hair (Natural hair) and the transplanted regenerated hair (Bio-hair) in the 1st and 2nd hair cycles. The error bars indicate the s.e.m. (n = 8 in natural hair, n = 5 in transplanted regenerated hair). (c) Histological and immunohistochemical analyses of the transplanted regenerated hair follicles at day 30 and day 42. H&E-staining (upper panels) and immunostaining with anti-GFP (lower panels) of a transplanted regenerated hair follicle of a serial section is shown. The boxed areas in each panel are shown at a higher magnification in the right panels. (d) Macroscopic observations of transplanted regenerated Awl hair (upper, at 1st and 2nd growth term) and zigzag hair (lower, at 1st and 2nd growth term). The right panels show macro-morphological images of each hair follicle at day 43 and day 49.