Table 1.
von Hippel Lindau genotype and Fuhrman grade in primary and metastatic CCRCC tumors.
| Pat. ID | Sample ID | Surgery date | Primary (P)/metastatic (M)/normal | VHL genotype | Fuhrman grade |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gr. 1/2 | Gr. 3/4 | |||||||||
| CATEGORY 1: PATIENTS WITH IDENTICAL PRIMARY AND METASTATIC VHL GENOTYPE IN TUMORS | ||||||||||
| 4 | 1 | 12/2004 | Primary, left kidney (P) | WT | Not evaluated | |||||
| 4 | 2 | 12/2004 | Metastatic, lymph node (M) | WT | Not evaluated | |||||
| 5 | 3 | 8/2002 | Primary, left kidney (P) | 478delG, ex3 | 0 | 100 | ||||
| 5 | 4 | 8/2002 | Metastatic, lymph node (M) | 478delG,ex3 | 0 | 100 | ||||
| 5 | 5 | 8/2002 | Adjacent normal | WT | ||||||
| 8 | 6 | 2/2005 | Primary, right kidney (P) | Methylated Promoter | 20 | 80 | ||||
| 8 | 7 | 2/2005 | Metastatic, adrenal (M) | Methylated Promoter | 0 | 100 | ||||
| 9* | 8 | 8/2003 | Primary, left kidney (P) | 232delA,ex1 | 10 | 90 | ||||
| Renal tumor | ||||||||||
| 9 | 9 | 8/2003 | Primary, left kidney (P) | 232delA,ex1 | 89 | 11 | ||||
| Tumor close to capsule | ||||||||||
| 9* | 10 | 8/2003 | Metastatic, lymph node (M) | 232delA, ex1 | 95 | 5 | ||||
| 11 | 11 | 2/2004 | Primary, left kidney (P) | 349delT,ex1 | 10 | 90 | ||||
| 11 | 12 | 2/2004 | Metastatic, small bowel (M) | 349delT,ex1 | 50 | 50 | ||||
| 11 | 13 | 2/2004 | Adjacent normal | WT | ||||||
| 13 | 14 | 3/2004 | Primary, right kidney (P) | WT | 100 | 0 | ||||
| 13 | 15 | 3/2004 | Metastatic, left adrenal (M) | WT | 99 | 1 | ||||
| 13 | 16 | 3/2004 | Adjacent normal | WT | ||||||
| CATEGORY 2: PATIENTS WITH DIFFERENT PRIMARY AND METASTATIC VHL GENOTYPE IN TUMORS | ||||||||||
| 6* | 17 | 5/2003 | Primary, left kidney (P) | 407insATATATAT, ex2 | 100 | 0 | ||||
| 6* | 18 | 5/2004 | Metastatic, fallopian tube (M) | WT | 100 | 0 | ||||
| 6* | 19 | 5/2004 | Metastatic, fallopian tube (M) | WT | 100 | 0 | ||||
| 6* | 20 | 5/2004 | Metastatic fallopian tube (M) | 407insATATATAT, ex2 | 100 | 0 | ||||
| 6 | 21 | 5/2003 | Adjacent normal | WT | ||||||
| 7* | 22 | 8/2004 | Primary, left kidney (P) | WT | 100 | 0 | ||||
| 7* | 23 | 8/2004 | Metastatic, colon (M) | G463C, ex2 | 0 | 100 | ||||
| 10* | 24 | 9/2002 | Primary, right kidney (P) | WT | 90 | 10 | ||||
| Renal vein margin with tumor | ||||||||||
| 10* | 25 | 9/2002 | Primary, right kidney (P) | C333G, ex1 | 10 | 90 | ||||
| Spatially separated representative section of primary tumor | ||||||||||
| 10* | 26 | 12/2003 | Primary, left kidney (P) | C333G, ex1 | 45 | 55 | ||||
| Representative section of tumor in relation to capsular margin | ||||||||||
| 10* | 27 | 12/2003 | Primary, left kidney (P) | C333G, ex1 | 66 | 34 | ||||
| Representative section of tumor in relation to parenchymal margin | ||||||||||
| 10* | 28 | 12/2003 | Metastatic, lymph node (M) | C333G, ex1 | 100 | 0 | ||||
| 10 | 29 | 9/2002 | Adjacent normal | WT | ||||||
| 10 | 30 | 12/2003 | Adjacent normal | WT | ||||||
| 10 | 31 | 12/2003 | Adjacent normal | WT | ||||||
| 12 | 32 | 3/2004 | Primary, right kidney (P) Representative section of closest parenchymal margin including area with renal sinus bulge of tumor |
del31 bp, intron 1, 9nt before ex2 | 95 | 5 | ||||
| 12 | 33 | 3/2004 | Primary, right kidney (P) Representative section of closest parenchymal margin including area with renal sinus bulge of tumor |
del31 bp in intron 1, 9nt before ex2 | 95 | 5 | ||||
| 12 | 34 | 3/2004 | Primary, right kidney (P) Representative section of same primary |
del31 bp in intron 1, 9nt before ex2 | 95 | 5 | ||||
| 12* | 35 | 4/2004 | Primary, left kidney (P) | WT | 90 | 10 | ||||
| Sections of tumor with capsule | ||||||||||
| 12 | 36 | 4/2004 | Primary, left kidney (P) | WT | 50 | 50 | ||||
| Same primary | ||||||||||
| 12* | 37 | 3/2004 | Lung metastasis | WT | 10 | 90 | ||||
| Parenchymal line of resection | ||||||||||
| 12* | 38 | 3/2004 | Lung metastatsis | WT | 10 | 90 | ||||
| Pleural margin of tumor | ||||||||||
| 12 | 39 | 3/2004 | Lung metastasis | WT | 10 | 90 | ||||
| Remaining metastatic tissue | ||||||||||
| 12 | 40 | 3/2004 | Adjacent normal | WT | ||||||
| 12 | 41 | 3/2004 | Adjacent normal | WT | ||||||
| 12 | 42 | 3/2004 | Adjacent normal | WT | ||||||
All nucleotide positions are numbered with the adenosine of the AUG start site as position number 1. This corresponds to nt position 214 in the mRNA sequence GenBank accession no. NM_000551. DNA sequencing traces are included in Appendix.
*Samples that were also sent to Transgenomic.