Figure 5.

βPix expression in kidney cells lines. Western blot analysis of cultured kidney cells reveals the ubiquitous expression of βPix. The data presented for proteins extracted from immortalized human mesangial cells (HM), human renal carcinoma 786-O cells (AC), immortalized mouse cortical collecting duct principal cells (mpkCCD_c14 and M-1), Madin–Darby canine kidney cells (MDCK) and renal proximal tubule epithelial cells (RTEC). Equal amount of protein loaded in each lane was verified by western blot with anti-β-actin antibodies (reproduced from Pavlov et al., 2010 with permission).