Table 1.
Primer | Sequence (5′-3′) | |
---|---|---|
gvcH-RT-F | TAAAGATCCAGCCACCG | |
gvcH-RT-R | GGCATTACTGAACACGC | |
ramA-RT-F | CGATTGTCGAGTGGATT | |
ramA-RT-R | GCGTAAAGGTTTGCTGC | |
csgA-RT-F | CGACCATTACCCAGAGC | |
csgA-RT-R | TTGCCAAAACCAACCTG | |
cheM.-RT-F | GCCAGATTACGCACCTC | |
cheM-RT-R | TGCCAGCATGGAACAAC | |
yhcQ-RT-F | ATTCCCCTGCTGCTCGT | |
yhcQ-RT-R | ATGTCGTCGCTATTGCC | |
motA-RT-F | GTCTGCTGCTGGTTTGG | |
motA-RT-R | ATAGGGGCGTTCATTGT | |
motB-RT-F | CTTTTGGCGATGTGGGT | |
motB-RT-R | CAGCAGGGTGAAGTGGA | |
STM0347-RT-F | GGCATCGCTTCACTCTT | |
STM0347-RT-R | TCACCGACCGCTACATC | |
rpsS-RT-F | ATAAGTACGAGTCGGTGCG | |
rpsS-RT-R | CACTTGCTGAAGAAGGTAGA | |
16S-F | TTCCTCCAGATCTCTCTACGCA | |
16S-R | GTGGCTAATACCGCATAACG | |
fimA-RT-F | ACTATTGCGAGTCTGATGTTTG | |
fimA-RT-R | CGTATTTCATGATAAAGGTGGC | |
fimZ-RT-F | ATTCGTGTGATTTGGCGT | |
fimZ-RT-R | ACTTATCCTGTTGACCTT | |
fimY-RT-F | GAGTTACTGAACCAACAGCT | |
fimY-RT-R | GCCGGTAAACTACACGATGA | |
fimW-RT-F | AAAGTGAAAGTAAAGCGG | |
fimW-RT-R | AAGAGATAGATAATGCCG | |
stbC-F | ATACGGGATCCCG-GCTGACAAACAGGCTGGTGATAAACAAT 3′ | The underlined sequence denotes the BamHI restriction site |
stbC-R | CTACGGGATCCCG-TGACGGGCTAGGTAAACCTGATAATCTG 3 | The underlined sequence denotes the BamHI restriction site |