Table 1.
Concentration of extracts (μg/mL) |
α-amylase activity % inhibition (−) or stimulation (+)a |
|||||
---|---|---|---|---|---|---|
Crude water extract | Crude methanol Extract | Dichloro-methane fraction | Ethyl acetate fraction | n-Butanol fraction | Water fraction | |
62.5 | (−28.53 ± 1.31*) [−40.78 ± 3.56*] |
(−36.26 ± 3.64*) [−48.29 ± 3.65*] |
(−43.22 ± 0.59*) [−33.59 ± 5.61*] |
(−46.23 ± 5.63*) [−56.14 ± 1.16*] |
(−42.29 ± 1.61*) [−28.39 ± 0.64*] |
(−27.03 ± 1. 56*) [−37.98 ± 2.64*] |
125 | (−52.56 ± 2.15*) [−61.25 ± 2.86*] |
(−48.56 ± 2.25*) [−50.16 ± 2.68*] |
(−59.36 ± 4.56*) [−42.29 ± 3.34*] |
(−62.36 ± 7.62*) [−67.84 ± 3.75*] |
(−64.56 ± 6.53*) [−33.14 ± 1.23*] |
(−65.23 ± 3.47*) [−47.34 ± 3.21*] |
250 | (−64.54 ± 3.45*) [−65.29 ± 7.21*] |
(−53.12 ± 8.58*) [−58.23 ± 6.89*] |
(−60.24 ± 6.42*) [−50.17 ± 2.65*] |
(−78.36 ± 5.26*) [−68.59 ± 7.64*] |
(−74.31 ± 4.28*) [−38.49 ± 8.76*] |
(−72.56 ± 3.58*) [−63.95 ± 9.56*] |
500 | (−75.34 ± 5.68*) [−71.28 ± 6.41*] |
(−63.32 ± 4.54*) [−60.59 ± 11.74*] |
(−67.52 ± 5.98*) [−57.23 ± 10.94*] |
(−83.31 ± 6.14*) [−74.19 ± 9.34*] |
(−79.45 ± 9.36*) [−57.28 ± 1.87*] |
(−77.15 ± 4.51*) [−68.34 ± 10.31*] |
1000 | (−87.54 ± 4.56*) [−72.39 ± 9.61*] |
(−70.12 ± 7.87*) [−65.36 ± 8.53*] |
(−70.54 ± 8.61*) [−61.86 ± 6.75*] |
(−93.12 ± 6.53*) [−83.65 ± 7.86*] |
(−87.16 ± 9.86*) [−63.18 ± 9.65*] |
(−80.14 ± 7.82*) [−70.64 ± 12.15*] |
2000 | (−90.63 ± 6.89*) [−73.59 ± 11.83*] |
(−78.26 ± 9.34*) [−71.69 ± 13.45*] |
(−80.23 ± 10.08*) [−67.14 ± 12.34*] |
(−95.26 ± 10.18*) [−87.54 ± 12.37*] |
(−90.16 ± 8.95*) [−72.35 ± 11.89*] |
(−89.26 ± 13.56*) [−72.12 ± 13.24*] |
aResults are expressed as mean percentage ± S.E.M of three observations in each group; (% inhibition of AM); (% inhibition of FF).
Amylase inhibitory activity (%) was defined as the percentage decrease in maltose production rate over the control (without extract).
*Values significantly different (P < 0.05) from the control in each group without the extract added.