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Effect of tryptase on the VEGF, Flt-1, and Flk-1 mRNA levels in HDMECs. Different concentrations of tryptase (0, 1, and 10 nmol/L) were added into HDMECs for 6 h. The mRNA levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Real-time RT-PCR and normalized to GAPDH. The heparin control was also analyzed. *P < 0.05 compared to the group of heparin control.
