. 2012 May 15;2012:941465. doi: 10.5402/2012/941465

Table 1.

Primer pairs used to amplify PCR products.

Gene	Sequence (5′-3′)	Product size	Annealing T (°C)	GeneBank no.
VEGF	Forward: CAACATCACCATGCAGATTATGC	132 bp	60°C	NM_001033756
VEGF	Reverse: CCCACAGGGATTTTCTTGTCTT	132 bp	60°C	NM_001033756
Flt-1	Forward: TGGCTGCGACTCTCTTCTG	118 bp	60°C	NM_002019
Flt-1	Reverse: CAAAGGAACTTCATCTGGGTCC	118 bp	60°C	NM_002019
Flk-1	Forward: GGCCCAATAATCAGAGTGGCA	104 bp	60°C	NM_002253
Flk-1	Reverse: TGTCATTTCCGATCACTTTTGGA	104 bp	60°C	NM_002253
GAPDH	Forward: CATGAGAAGTATGACAACAGCCT	113 bp	60°C	NM_002046
GAPDH	Reverse: AGTCCTTCCACGATACCAAAGT	113 bp	60°C	NM_002046