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Biofilm-forming ability of isogenic wild-type, Caecm33 mutant, and revertant strains. (a) Metabolic activity of mature biofilms formed by the isogenic strains. (b–d) Fluorescence micrographs showing differences in morphology of biofilms formed by (b) wild-type, (c) ECM33 mutant, or (d) revertant strain. RML1-heterozygous Caecm33/CaECM33 mutant; RML2-homozygous Caecm33 mutant; RML3-heterozygous Caecm33/CaECM33 revertant; RML4-homozygous ECM33/ECM33 revertant.
