Figure 7.
Lung leukocyte composition before and after P. aeruginosa infection and neutrophil function. Control, WT BMT, and PTEN CKO BMT mice were injected intratracheally with 50μL of 5 × 105 CFU P. aeruginosa. Lung samples were harvested from uninfected and infected mice 24 hours later, and lung leukocytes were isolated, counted, and stained for differential cell analysis as described in Materials and Methods. (A and B) Total lung leukocyte count in naive (A) and infected (B) mice. (C and D) Percentage of macrophages/monocytes, neutrophils, lymphocytes, and eosinophils in the lungs of naive (C) and infected (D) mice (n = 4 per group). Neutrophils were elicited to the lung and harvested by BAL from control, WT BMT, and PTEN CKO BMT mice as described in Materials and Methods. Killing of serum-opsonized P. aeruginosa was assessed in neutrophils (E) as described in Materials and Methods (n = 7–10 per group; * P < 0.05, *** P < 0.001).