Fig. 2.
The catalytic activity of Dcs1 is not required for activation of TIF51A mRNA degradation. DCS1H-N denotes the catalytically inactive DCS1 mutant harboring a H268N substitution in its HIT motif (4). Degradation of TIF51A mRNA was monitored after transcriptional shutoff with thiolutin (15 μg/mL), and RNA was isolated at the indicated time points. (A) Northern blot showing degradation in dcs1Δ and/or dcs2Δ mutant backgrounds. The ScR1 RNA served as a loading control. (B) Half-life measurements of TIF51A transcripts were carried out by quantification of the mRNA remaining at each time point after correcting for loading differences using ScR1 RNA. The average value and SDs were obtained from at least three independent experiments.