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. 2012 May 7;109(21):8316–8321. doi: 10.1073/pnas.1200407109

Fig. 4.

Fig. 4.

Activity of MtVAMP721d and MtVAMP721e promoters. The 2.5-kb region upstream of the translational start of MtVAMP721d (A and C) and of MtVAMP721e (B, D, and E), respectively, were fused to GUS and transgenic roots expressing these genetic constructs were inoculated by S. meliloti 2011 (C and D) or G. intraradices (E). Nodules were harvested 14 dpi and were analyzed for GUS activity. Nodules were hand-sectioned. Promoters of MtVAMP721d and MtVAMP721e are active in the meristem and the infection zone of the nodule where release of bacteria from the infection threads and symbiosome development occurs. Mycorrhized roots were harvested and analyzed for GUS activity 28 dpi. Promoter MtVAMP721e shows activity in vascular tissue and arbuscule-containing cells (E, arrowheads). (Scale bars, 100 μm in A–E).