Fig. 1.

Hormonal regulation of uterine epithelial KLF4 and KLF15 expression. (A) Regulation of KLF4 and KLF15 protein expression by E₂ and P₄E₂, respectively. Equivalent amount of protein lysate isolated from uterine epithelial cells of mice treated with hormones as indicated were separated by SDS PAGE and subjected to Western blotting with specific antibodies as shown. Actin was used as a protein loading control. (B) Cross section of the midportion of the uterus immunostained using antibodies against KLF4 (i, iii) or KLF15 (ii, iv) 3 h after corresponding hormone treatment or with control IgG (v, vi). Brown staining indicates positive reactivity. The data described here are representative of the analysis of at least 5 different mice for each group. Scale bar: 50 um. (C) Regulation of KLF4, KLF15 protein expression 3 h after E₂ and P₄E₂ treatment in uterine epithelial and stromal compartments. Equivalent amount of protein lysate isolated from uterine epithelial or stromal cells of mice treated with hormones as indicated were separated by SDS PAGE and subjected to Western blotting with specific antibodies as shown. Actin was used as a protein loading control.