Fig. 3.

Temporal binding patterns of KLF4 and KLF15 compared to RNA Pol II on the Mcm2 promoter. (A) Analysis of recruitment of ERα, PR, KLF-4, KLF-15, and RNA Pol II by ChIP on the uterine Mcm2 promoter 14 h after hormone treatment. The results presented are compared to input and normalized against IgG control. (B) Correlation between KLF15 recruitment and RNA Pol II recruitment 14 h after E₂ (i) and P₄E₂ (ii) treated samples. Shown are representative bar diagrams that established a reciprocal relationship between KLF15 and RNA Pol II association. (Differences between binding ***p < 0.001, n = 5). (C) Identification of a direct link between KLF15 and RNA Pol II association by comparing 3 h vs. 14 h in the P₄E₂ treated samples at four discrete positions on the Mcm2 promoter. In each case a reduction in KLF15 binding at 14 h is correlated with an increase in RNA Pol II binding. Differences in binding at each position ***p < 0.001, n = 5. Bar identification shown in figure is as described in Fig. 2.