Normalized concentration of each component, C5/Ctotal (A), (B), A/Atotal (C) plotted against the inverse temperature β. (A and B) Maximum (red), minimum (green), and average (blue) over a cycle. (A) Note that the maximum value of C5 is nearly constant, whereas its minimum and average increase with β beyond βc (i.e., at lower temperature). (B) The average value of is fitted well by the value of the unstable fixed point of the equation (magenta line), which is proportional to exp(-β(Ep - Edp). (C) The concentration of free KaiA in the plateau region of C5 is plotted, as estimated from the time where C3 reaches a peak. This free KaiA concentration closely follows exp(βEp) (magenta line), for β > βc—i.e., below the critical temperature.