Fig. 1.

Functionally different ligands trigger different GHS-R1a conformations. (A) Fluorescence emission spectra of bimane attached to C¹⁴⁶ in the absence of ligand or in the presence of ghrelin, of JMV 3018, or of SPA. (B) Fluorescence emission spectra of bimane attached to C³⁰⁴ in the absence of ligand or in the presence of ghrelin or of JMV 3018. (C) Fluorescence emission spectra of bimane attached to C³⁰⁴ in the absence of ligand or in the presence of SPA. (D and E) Percentage changes in maximum emission intensity induced by functionally different ligands in the absence of protein effector. The data are representative of two experiments performed in duplicate, and the error bar represents the SEM.