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Effector proteins affect agonist-induced GHS-R1a conformations. (A and C) Fluorescence emission spectra of bimane attached to C304 in the presence of ghrelin (A) or JMV3018 (C) and in the absence or in the presence of effector proteins. Similar profiles were obtained with bimane attached to C146 (SI Appendix, Fig. S15). (B and D) Percentage changes in maximum emission intensity in the presence of ghrelin (B) or JMV3018 (D) and in the absence or in the presence of effector proteins.
