Table 2.

Characteristics and performance data of the primers used for reference gene-stability measure M and quantitative real-time PCR analysis

Gene	Forward primer (from 5' to 3')	PCR product	NCBI GenBank	Slope	R2	Efficiency	M
	Reverse primer (from 5' to 3')
Reference genes
ACTB	ACTTGCGCAGAAAACGAGAT	120	AY141970	-0.30	0.99	1.99	0.039
	CACCTTCACCGTTCCAGTTT
SDHA	GCAGAACCTGATGCTTTGTG	185	NM_174178	-0.24	0.99	1.74	0.048
	CGTAGGAGAGCGTGTGCTT
ATP5B	GGACTCAGCCCTTCAGCGCC	229	NM_175796.2	-0.16	0.99	1.44	0.039
	GCCTGGTCTCCCTGCCTTGC
RPS9	GTGAGGTCTGGAGGGTCAAA	108	BC148016	-0.31	0.99	2.04	0.040
	GGGCATTACCTTCGAACAGA
PPIA	GGCAAATGCTGGCCCCAACACA	87	NM_178320.2	-0.34	0.99	2.13	0.034
	AGTACCACGTGCTTGCCATCCA
RPL12	CACCAGCCGCCTCCACCATG	84	NM_205797.1	-0.35	0.99	2.25	0.036
	CGACTTCCCCACCGGTGCAC
Target genes
ACADM	GCGAGTACCCTGTCCCATTA	243	NM_001075235	-0.29	0.99	1.93
	CCTCAGTCATTCTCCCCAAA
ACOX1	CCATTGCCGTCCGATACAGT	99	BC102761	-0.27	0.96	1.88
	GTTTATATTGCTGGGTTTGATAATCCA
ALDH9A1	CAGGATTCGGCAGAGAGAAC	229	NM_001046423	-0.28	0.99	1.90
	TGAGCCATGAAGAGCATCAC
BBOX1	TCCAGCTGCCTACTCTGGAT	292	BC149884.1	-0.28	0.99	1.91
	AGCTGAACCTTACCCCAGGT
CD36	GCATTCTGAAAGTGCGTTGA	179	BC103112	-0.28	0.98	1.91
	CGGGTCTGATGAAAGTGGTT
CPT1A	CAAAACCATGTTGTACAGCTTCCA	111	FJ415874	-0.32	0.99	2.09
	GCTTCCTTCATCAGAGGCTTCA
HMGCS2	GCCCAATATGTGGACCAAAC	209	NM_001045883	-0.29	0.99	1.96
	ATGGTCTCAGTGCCCACTTC
PPARA	GGTGGAGAGTTTGGCAGAACCAGA	168	BT020756.1	-0.23	0.99	1.70
	TCCCACTGCCCAGCTCCGATC
SLC22A5	CACAGTGGTCAGGAACATGG	181	BC105377	-0.28	0.99	1.89
	AATGGTGTCTGGGAGTGGAG
SLC27A1	CTGAAGGAGACCTCCACAGC	208	NM_001033625.2	-0.30	0.99	1.99
	GTGGTACAGGGGCAGACAGT
TMLHE	TGGCAGGACACTGCTAGTTG	222	NM_001076064.1	-0.31	0.99	2.05
	GACAGCCCGGTCATAGTTGT