. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Cytokine. 2011 Nov 12;57(1):1–8. doi: 10.1016/j.cyto.2011.10.005

Table 1.

Functional repopulation potential of phenotypically defined HSC subsets.

Population	Phenotype	Functional repopulation activity^*	Comments	Citation
Mouse
HSPC	lin⁻Sca-1+c-kit⁺ (LSK)	~1 in 100	Heterogeneous, contains self-renewing and non-renewing subsets	[64,65]
HSC	flk2/flt3⁻ LSK	~1 in 50	Can be further enriched for HSC activity using VCAM-1	[66–68]
HSC	CD27⁻ LSK	~1 in 50	Does not capture all HSCs as some self-renewal activity is detectable within the CD27+ subset	[69]
HSC	CD150⁺CD48⁻ LSK	~1 in 2–5		[13]
HSC	SP (side population) LSK	~1 in 2–5		[70,71]
HSC	Thy-1.1^loSca-1⁺ LSK	1 in 5–22	Thy-1 and Sca-1 not conserved across mouse strains	[13,72–74]
Human
HSC	CD34⁺CD38⁻	1 in 617		[15]
HSC	CD133⁺ALDH^hilin⁻	1 in 691		[16]
HSC	CD34⁺CD38⁻CD45RA⁻Thy1⁺CD49f⁺	1 in 10.5		[18]

As measured by the ability to sustain multi-lineage hematopoiesis long-term, usually 16 weeks, after transplantation to an immune deficient recipient.