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. 2012 Feb 17;6(3):276–283. doi: 10.1016/j.molonc.2012.02.002

Figure 2.

Figure 2

E804 reduces levels of p‐Stat5 (Y694) and inhibits Stat5 DNA‐binding activity in CML cells. A. K562, KCL‐22M and CD34‐positive primary CML cells were treated with E804 in a dose‐dependent manner for 4 h. Whole‐cell lysates were immunoblotted with specific antibodies to p‐Stat5 (Y694) and total Stat5 (top and middle panels). For EMSA analysis (bottom panel), nuclear extracts from CML cells treated with E804 were prepared as in Figure 1. B. K562 CML cells were treated with 10 μM of E804 in a time‐dependent manner. Whole‐cell lysates were immunoblotted with specific antibodies to p‐Stat5 (Y694) and total Stat5 (top panel). EMSA analysis was conducted as in Figure 1B (bottom panel). Supershift was done with antibody to Stat5 as indicated.