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. 2012 Feb 17;6(3):276–283. doi: 10.1016/j.molonc.2012.02.002

Figure 5.

Figure 5

E804 down‐regulates anti‐apoptotic Mcl‐1 and Bcl‐xL proteins, associated with reduction of cell viability and induction of apoptosis in K562 CML cells. A. K562 CML cells were treated with E804 in a dose‐dependent manner for 48 h. Whole‐cell lysates were immunoblotted with specific antibodies to Mcl‐1, Bcl‐xL and β‐Actin. B. K562 CML cells were treated with E804 in a dose‐ or time‐dependent manner. Cell viability was determined using MTS assays as described in Materials and Methods. Each experiment was performed in quadruplicate. Data are mean ± SD. C and D. E804 induces apoptosis. Human K562 (C) and KCL‐22M (D) CML cells were treated with E804 in a dose‐dependent manner for 48 h. To determine induction of apoptosis, Annexin V‐FITC staining was used as an early marker of apoptosis. Each experiment was performed in quadruplicate. Data are mean ± SD.