E2 decreased expression of orexigenic genes in the posterior hypothalamus. (A) E2 decreased expression of AgRP in young and middle-aged rats. (B) E2 decreased expression of NPY in young and middle-aged rats. Regardless of hormone, NPY expression was higher in middle-aged than in young rats. (C, D) There was a main effect of hormone on MCH and orexin expression. E2 decreased expression of MCH and orexin in young and middle-aged rats. Data are expressed as mean ± SEM. RT-PRC values are expressed as fold change relative to young, oil-treated control *<control, P< 0.05. +> young, P< 0.05.