Table 1.

Primer Sequences Used for RT-PCR

Gene	Sense	Antisense
CRH	5’GCT GTC CCC CAA CTC CAC	5’ CAG CTC CGT GCT GCT GTC
POMC	5’ CTC CAT AGA CGT GTG GAG CTG	5’ TCA GTC AAG GGC TGT TCA TCT
Lepr	5’AGG GAA CCT GTG AGG ATG AGT	5’ TGT CTC AGT GGG GAA TGT TTC
5HT2CR	5’ AGA AAG AAA AGC GTC CCA GAG	5’CCA CAA AGA ACC GAC AGG ATA
AgRP	5’TGA AGA AGA CAG CAGCAG ACC	5’AAG GTA CCT GTT GTC CCA AGC
NPY	5’GTA ACA AAC GAA TGG GGC TGT	5’ CGC AGA GCG GAG TAG TAT CTG
pMCH	5’ ATG CTG GCC TTT TCT TTG TTT	5’ AAG GAG CAA CAA CCG ATC TTT
Orexin	5’GCA TCC TCA CTC TGG GAA AG	5’ GCA GGG ATA TGG CTC TAG CTC
β-actin	5’AGA TTA CTG CCC TGG CTC CTA	5’CTC ATC GTA CTC CTG CTT GCT

Abbreviations: CRH = corticotropin releasing hormone; POMC = proopiomelanocortin; Lepr = long form of the leptin receptor; 5HT2CR = serotonin 2C receptor; AgRP = agouti-related peptide; NPY = neuropeptide Y; pMCH = prepromelanin-concentrating hormone.