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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Bone Miner Res. 2012 May;27(5):1030–1042. doi: 10.1002/jbmr.1553

Figure 1.

Figure 1

A. pOBCOL2.3-hIL-7 construct. pOBCOL2.3-hIL-7 contains 2300bp of 5’flanking sequence of the rat col1α1 promoter, first exon and first intron driving human IL-7 cDNA. B. RT-PCR analysis of transgene expression. Selected tissues from pOBCOL2.3-hIL-7 transgenic mouse (high Tg Line C). Total RNA was extracted from 6 week old heterozygous female mouse. Ten micrograms of total RNA was reverse transcribed and 1/50 of cDNA mixture was used for PCR reaction for human IL-7 (hIL-7) and mouse actin.