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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Bone Miner Res. 2012 May;27(5):1030–1042. doi: 10.1002/jbmr.1553

Figure 5. T cell lymphopoiesis remains deficient in IL-7 KO mice after selective expression of IL-7 by osteoblasts.

Figure 5

Cell suspensions from thymus and spleen from WT, Tg IL-7, IL-7 KO and rescued IL-7 KO mice were stained with anti-CD3, anti-TCRαβ and anti-TCR-γδ antibodies coupled to distinct fluorochromes, and analyzed by flow cytometry as described. Histograms on the left showed the percentage of cells reactive to anti-CD3 and plots in the right show the distribution of TCRαβ and TCRγδ within CD3 gated populations.